Title :
An ISFET based analogue ratiometric method for DNA methylation detection
Author :
Kalofonou, Melpomeni ; Toumazou, Christofer
Author_Institution :
Dept. of Electr. & Electron. Eng., Inst. of Biomed. Eng., London, UK
Abstract :
This paper presents the concept of a ratiometric approach for DNA methylation detection using the “Methylation Cell” for the indication of epigenetic abnormalities related to cancer. The “Methylation Cell” allows real-time detection of pH signals resulting from DNA based reactions using ISFET sensors, performing continuous computation of the ratio of DNA methylation, giving a discrete output signal when DNA methylation exceeds a certain predefined percentage. Fabricated in a typical 0.35μm CMOS process, it uses current-mode translinear circuits to perform computation in low power. Experimental results are presented, demonstrating its capabilities through an integration in a Lab-on-Chip (LoC) set-up using a microfluidic assembly.
Keywords :
DNA; bioMEMS; biochemistry; cancer; cellular biophysics; electrochemical sensors; genetics; ion sensitive field effect transistors; lab-on-a-chip; microfluidics; microsensors; pH; CMOS process; DNA based reactions; DNA methylation detection; ISFET based analogue ratiometric method; ISFET sensors; Lab-on-Chip set-up; LoC; Methylation Cell; cancer; continuous computation; current-mode translinear circuits; discrete output signal; epigenetic abnormalities; low power computation; microfluidic assembly; pH signals; real-time detection; CMOS integrated circuits; Cancer; Chemical sensors; Chemicals; DNA; Sensors; DNA methylation; ISFET; Lab-on-Chip; translinear;
Conference_Titel :
Circuits and Systems (ISCAS), 2014 IEEE International Symposium on
Conference_Location :
Melbourne VIC
Print_ISBN :
978-1-4799-3431-7
DOI :
10.1109/ISCAS.2014.6865514
