Title :
Two-photon fluorescence resonance energy transfer stoichiometry in living cells
Author :
Bhagwat, Amar R. ; Flynn, Damian ; Brenner, Meredith H. ; Nunez, Marcos ; Ogilvie, Jennifer P. ; Dawen Cai ; Swanson, Joel A.
Author_Institution :
Dept. of Phys., Univ. of Michigan, Ann Arbor, MI, USA
Abstract :
Using phase-shaped pulses, we perform, for the first time, a proof-of-principle demonstration of two-photon fluorescence resonance energy transfer (FRET) microscopy for studying the stoichiometry of intermolecular interactions within living cells.
Keywords :
cellular biophysics; fluorescence; molecular biophysics; optical microscopy; stoichiometry; two-photon processes; FRET microscopy; intermolecular interactions; living cells; phase-shaped pulses; proof-of-principle demonstration; stoichiometry; two-photon fluorescence resonance energy transfer microscopy; Cells (biology); Educational institutions; Energy exchange; Fluorescence; Laser excitation; Microscopy; Optical filters;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2014 Conference on
Conference_Location :
San Jose, CA
