DocumentCode
1769438
Title
Two-photon fluorescence resonance energy transfer stoichiometry in living cells
Author
Bhagwat, Amar R. ; Flynn, Damian ; Brenner, Meredith H. ; Nunez, Marcos ; Ogilvie, Jennifer P. ; Dawen Cai ; Swanson, Joel A.
Author_Institution
Dept. of Phys., Univ. of Michigan, Ann Arbor, MI, USA
fYear
2014
fDate
8-13 June 2014
Firstpage
1
Lastpage
2
Abstract
Using phase-shaped pulses, we perform, for the first time, a proof-of-principle demonstration of two-photon fluorescence resonance energy transfer (FRET) microscopy for studying the stoichiometry of intermolecular interactions within living cells.
Keywords
cellular biophysics; fluorescence; molecular biophysics; optical microscopy; stoichiometry; two-photon processes; FRET microscopy; intermolecular interactions; living cells; phase-shaped pulses; proof-of-principle demonstration; stoichiometry; two-photon fluorescence resonance energy transfer microscopy; Cells (biology); Educational institutions; Energy exchange; Fluorescence; Laser excitation; Microscopy; Optical filters;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics (CLEO), 2014 Conference on
Conference_Location
San Jose, CA
Type
conf
Filename
6988332
Link To Document