Title :
Novel wedge-based approach for simultaneous multichannel microscopy
Author :
Chung, Samuel H. ; Gabel, Christopher V.
Author_Institution :
Phys. Sci. Inc., Andover, MA, USA
Abstract :
We demonstrate a novel device, based on wedge prisms, that enables simultaneous imaging and fluorescence microscopy of multiple color channels and is simpler, more user-friendly, and less expensive than current commercial devices. Applications include ratiometric calcium imaging and co-localization of multiple labels.
Keywords :
fluorescence; optical microscopy; commercial devices; fluorescence microscopy; multiple color channels; multiple label colocalization; ratiometric calcium imaging; simultaneous multichannel microscopy; wedge prisms; wedge-based approach; Calcium; Charge coupled devices; Fluorescence; Microscopy; Optical filters; Proteins;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2014 Conference on
Conference_Location :
San Jose, CA
