Title :
Nonlinear Fourier-transform spectroscopy using ultrabroadband pulses for measurement of photobleaching spectra of fluorescent proteins
Author :
Suda, Akira ; Takahashi, Hiroki ; Toda, Keisuke
Author_Institution :
Dept. of Phys., Tokyo Univ. of Sci., Noda, Japan
Abstract :
We present the measurement of photobleaching spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy using ultrabroadband femtosecond pulses. Photobleaching of two-photon excited fluorescent molecules occurs through one-photon excited-state absorption.
Keywords :
Fourier transform spectra; fluorescence; molecular biophysics; optical saturable absorption; proteins; two-photon spectra; fluorescent proteins; nonlinear Fourier-transform spectroscopy; one-photon excited-state absorption; photobleaching spectra; two-photon excited fluorescent molecules; ultrabroadband femtosecond pulses; Delays; Fluorescence; Fourier transforms; Optical interferometry; Photobleaching; Proteins; Spectroscopy;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2014 Conference on
Conference_Location :
San Jose, CA
