DocumentCode
1771763
Title
Microfluidic flow cytometer for multiparameter screening of fluorophore photophysics
Author
Dean, Kevin M. ; Davis, Lloyd M. ; Lubbeck, Jennifer L. ; Manna, Premashis ; Palmer, Amy E. ; Jimenez, R.
Author_Institution
Dept. of Chem. & Biochem., Univ. of Colorado, Boulder, CO, USA
fYear
2014
fDate
8-13 June 2014
Firstpage
1
Lastpage
2
Abstract
We present a microfluidic cytometer that sorts mammalian or yeast cells by laser force deflection following real-time multibeam, multiparameter fluorescence measurements, including photobleaching, lifetime and expression level, of the intrinsic fluorophores within each cell.
Keywords
bioMEMS; biological techniques; cellular biophysics; fluorescence; microfluidics; optical saturable absorption; radiative lifetimes; fluorescence lifetime; fluorophore photophysics; intrinsic fluorophores; laser force deflection; mammalian cells; microfluidic flow cytometer; multiparameter fluorescence measurements; multiparameter screening; photobleaching; real-time multibeam; yeast cells; Fluorescence; Instruments; Laser beams; Measurement by laser beam; Microfluidics; Photobleaching; Proteins;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics (CLEO), 2014 Conference on
Conference_Location
San Jose, CA
Type
conf
Filename
6989594
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