Title :
Microfluidic flow cytometer for multiparameter screening of fluorophore photophysics
Author :
Dean, Kevin M. ; Davis, Lloyd M. ; Lubbeck, Jennifer L. ; Manna, Premashis ; Palmer, Amy E. ; Jimenez, R.
Author_Institution :
Dept. of Chem. & Biochem., Univ. of Colorado, Boulder, CO, USA
Abstract :
We present a microfluidic cytometer that sorts mammalian or yeast cells by laser force deflection following real-time multibeam, multiparameter fluorescence measurements, including photobleaching, lifetime and expression level, of the intrinsic fluorophores within each cell.
Keywords :
bioMEMS; biological techniques; cellular biophysics; fluorescence; microfluidics; optical saturable absorption; radiative lifetimes; fluorescence lifetime; fluorophore photophysics; intrinsic fluorophores; laser force deflection; mammalian cells; microfluidic flow cytometer; multiparameter fluorescence measurements; multiparameter screening; photobleaching; real-time multibeam; yeast cells; Fluorescence; Instruments; Laser beams; Measurement by laser beam; Microfluidics; Photobleaching; Proteins;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2014 Conference on
Conference_Location :
San Jose, CA
