Title :
In vivo three-photon calcium imaging of brain activity from layer 6 neurons in mouse brain
Author :
Ouzounov, Dimitre G. ; Horton, Nicholas ; Tianyu Wang ; Feng, D. ; Nishimura, Nozomi ; Xu, Changsheng
Author_Institution :
Sch. of Appl. & Eng. Phys., Cornell Univ., Ithaca, NY, USA
Abstract :
We demonstrate three-photon microscopy (3PM) at 1300-nm excitation for imaging neuronal activity as deep as layer-6 (L6) of adult mouse cortex using genetically-encoded calcium indicators. In vivo imaging of subcortical, GFP-labeled neurons is also demonstrated.
Keywords :
biomedical optical imaging; brain; multiphoton processes; neurophysiology; optical microscopy; GFP-labeled neurons; In vivo imaging; adult mouse cortex; genetical-encoded calcium indicators; in vivo three-photon calcium imaging; layer-6 neurons; mouse brain activity; subcortical neurons; three-photon microscopy; Brain; Calcium; In vivo; Mice; Microscopy; Neurons;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2014 Conference on
Conference_Location :
San Jose, CA
