Synthesizing genetic clock with toggle switch and oscillator

The potential of the genetic clock is in its role to trigger consecutive logic reactions in sequential biological circuits. However, it is hard to directly create a square wave generator in the gene regulatory systems for use as a genetic clock. This research studies the design of a genetic clock generator based on combination of a genetic oscillator with a toggle switch via a dual repressor. This connection is simple and direct in the structure. Realization of the biological circuit is easy as well. We adopt the design approach based on the real structured genetic algorithm (RSGA), controlling amplitude, phase, and frequency of the genetic oscillator in silico. Analysis of the genetic clock´s characteristics is conducted.