Title :
Development of an optical sensor for rapid and automatic gram classification of microorganisms
Author :
Hilding Ohlsson, Astrid ; Bonetto, M.C. ; Sacco, N.J. ; Corton, E.
Author_Institution :
Fac. de Cienc. Exactas y Naturales, Dept. de Quim. Biol., IQUIBICEN, Univ. de Buenos Aires, Buenos Aires, Argentina
Abstract :
Based on the classical Gram staining technique we created an adapted protocol so it could be used in bacterial suspensions for Gram positive and negative bacteria. The differences between these two types are determined by spectrophotometry. This is a rapid and low cost procedure to classify bacteria.
Keywords :
biological techniques; microorganisms; microsensors; protocols; spectrophotometry; suspensions; Gram negative bacteria; Gram positive bacteria; automatic Gram classification; bacterial suspension; classical Gram staining technique; microorganisms; optical sensor; protocol; spectrophotometry; Bleaching; Crystals; Fluorescence; Microorganisms; Optical sensors; Protocols; Suspensions; Gram; bacteria classification; rapid; sensor; staining;
Conference_Titel :
Sensors (IBERSENSOR), 2014 IEEE 9th Ibero-American Congress on
Conference_Location :
Bogota
Print_ISBN :
978-1-4799-6835-0
DOI :
10.1109/IBERSENSOR.2014.6995522
