Broadband Electrical Detection of Individual Biological Cells

Author

Yaqing Ning ; Multari, Caroline ; Xi Luo ; Palego, Cristiano ; Xuanhong Cheng ; Hwang, James C. M. ; Denzi, Agnese ; Merla, Caterina ; Apollonio, Francesca ; Liberti, Micaela

Author_Institution

Dept. of Electr. & Comput. Eng., Lehigh Univ., Bethlehem, PA, USA

Volume

62

Issue

9

fYear

2014

fDate

Sept. 2014

Firstpage

1905

Lastpage

1911

Abstract

To resolve the dilemma of cell clogging or solution parasitics encountered by Coulter counters and to evolve a general-purpose electrical detection technique, we used broadband microwave measurements to overcome electrode polarization, ac dielectrophoresis to precisely place cells between narrowly spaced electrodes, and relatively wide microfluidic channels to prevent cell clogging. This unique combination of approaches resulted in reproducible sensing of single Jurkat and HEK cells, both live and dead, of different cultures at different times.

Keywords

bioMEMS; bioelectric phenomena; biomedical electrodes; cellular biophysics; electric sensing devices; electrophoresis; microfluidics; microsensors; microwave measurement; Coulter counters; HEK cells; ac dielectrophoresis; biological cells; broadband electrical detection; broadband microwave measurements; cell clogging dilemma; electrode polarization; microfluidic channels; single Jurkat cells; solution parasitic dilemma; Broadband communication; Capacitance; Dielectrophoresis; Dispersion; Educational institutions; Electrodes; Impedance; Biological cells; biological engineering; biological liquids; biomedical transducers; microstrip; microwave measurements; sensitivity;

fLanguage

English

Journal_Title

Microwave Theory and Techniques, IEEE Transactions on

Publisher

ieee

ISSN

0018-9480

Type

jour

DOI

10.1109/TMTT.2014.2342660

Filename

6873335