Comparative study of DNA integrity detection with the assay comet in cells of whole blood and isolated mononuclear

Author

Arango-Varela, S.S. ; Tobon, C. ; Alvarez-Castro, A.O. ; Tobon-Coronado, C.A.

fYear

2014

fDate

12-14 Nov. 2014

Firstpage

1

Lastpage

4

Abstract

The comet assay is a tool to detect low levels of DNA damage and is applicable in almost all the cells of the body. In this study, the level of DNA damage between whole blood cells and peripheral blood mononuclear cells isolated from individuals selected was compared. All samples were obtained from 35 participants with coffee, alcohol and / or cigarettes consumption habits. Higher percentages of damage (% Tail Moment) in isolated mononuclear cells compared to whole blood samples were demonstrated. In this study there was no evidence that associate consumption of liquor, cigarette and coffee with loss of DNA integrity.

Keywords

DNA; biomedical measurement; blood; cellular biophysics; genetics; genomics; DNA damage level; DNA integrity detection; Tail Moment; alcohol consumption habits; assay comet; body cells; cigarette consumption habits; coffee consumption habits; comparative study; isolated mononuclear cells; liquor; peripheral blood mononuclear cells; whole blood cells; Blood; Cells (biology); DNA; Fluorescence; In vitro; Maintenance engineering; Software; Comet Assay; Mononuclear cells; genotoxicity; integrity DNA; whole blood;

fLanguage

English

Publisher

ieee

Conference_Titel

Central America and Panama Convention (CONCAPAN XXXIV), 2014 IEEE

Conference_Location

Panama City

Type

conf

DOI

10.1109/CONCAPAN.2014.7000400

Filename

7000400