Pinpoint chemical stimulation at a single-cell scale by microfluidic techology

Author

Horayama, Masayuki ; Ohkubo, Tomoki ; Arai, Kenta ; Kabayama, Kazuya ; Fujii, Teruya ; Kimura, Hiromitsu

Author_Institution

Tokai Univ., Hiratsuka, Japan

fYear

2014

fDate

10-12 Nov. 2014

Firstpage

1

Lastpage

2

Abstract

In this study, we have developed a microfluidic device to control chemical stimulation to a cell population at a single cell scale for cell-based assay. The device consists of a cell culture chamber and microfluidic probe (MFP) channels which are possible to control pinpoint chemical stimulation in the microspace. The device has been evaluated by a computer simulation using Finite Element Method (FEM) and a flow control experiment. A control of chemical stimulation from single cell scale to a cell population scale in the chamber can be possible by regulating flow ratio and channel size of the microfluidic probe.

Keywords

bioMEMS; biomechanics; cellular biophysics; finite element analysis; flow control; flow simulation; microchannel flow; FEM; cell culture chamber; cell population; cell-based assay; channel size; chemical stimulation; computer simulation; finite element method; flow control experiment; flow ratio; microfluidic device; microfluidic probe channels; microfluidic techology; pinpoint chemical stimulation; single-cell scale; Chemicals; Finite element analysis; Microfluidics; Ports (Computers); Probes; Sociology; Statistics;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2014 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4799-6678-3

Type

conf

DOI

10.1109/MHS.2014.7006096

Filename

7006096