Title :
Pinpoint chemical stimulation at a single-cell scale by microfluidic techology
Author :
Horayama, Masayuki ; Ohkubo, Tomoki ; Arai, Kenta ; Kabayama, Kazuya ; Fujii, Teruya ; Kimura, Hiromitsu
Author_Institution :
Tokai Univ., Hiratsuka, Japan
Abstract :
In this study, we have developed a microfluidic device to control chemical stimulation to a cell population at a single cell scale for cell-based assay. The device consists of a cell culture chamber and microfluidic probe (MFP) channels which are possible to control pinpoint chemical stimulation in the microspace. The device has been evaluated by a computer simulation using Finite Element Method (FEM) and a flow control experiment. A control of chemical stimulation from single cell scale to a cell population scale in the chamber can be possible by regulating flow ratio and channel size of the microfluidic probe.
Keywords :
bioMEMS; biomechanics; cellular biophysics; finite element analysis; flow control; flow simulation; microchannel flow; FEM; cell culture chamber; cell population; cell-based assay; channel size; chemical stimulation; computer simulation; finite element method; flow control experiment; flow ratio; microfluidic device; microfluidic probe channels; microfluidic techology; pinpoint chemical stimulation; single-cell scale; Chemicals; Finite element analysis; Microfluidics; Ports (Computers); Probes; Sociology; Statistics;
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2014 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4799-6678-3
DOI :
10.1109/MHS.2014.7006096
