Evaluation of enzyme immobilization methods on microglucose sensors integrated to a microfluidic device

This paper reports on development of a micro glucose sensor integrated-device for cell-based assays. Glucose is the energy source of living cells. Therefore, measuring the glucose consumption on cells is very important in cell biology. In μTAS research field, an enzyme-fixed micro glucose sensor integrated to microfluidic devices has been proposed for measurement of cell kinetics continuously. There are various methods on how to fix the enzyme to the sensor. In this paper, we examined the performance of the micro glucose sensor fabricated by the different enzyme immobilization methods. We measured the sensitivity and the lifetime of the glucose sensors made by different enzyme immobilization methods. As the result, the sensor sensitivity of the crosslinking method was higher than it of the physisorption method. We conclude that the performance of the micro glucose sensor could be modified by optimization of the enzyme immobilization methods.