Title :
Microassembly of spheroids by thermoresponsive gel probe
Author :
Takeuchi, Masaru ; Nakajima, Masahiro ; Fukuda, Toshio ; Hasegawa, Yohei
Author_Institution :
Dept. of Micro-Nano Syst. Eng., Nagoya Univ., Nagoya, Japan
Abstract :
In this paper, manipulation of cell spheroids was conducted to construct 3D cell structures for tissue engineering applications. The spheroids were manipulated using thermoresposnive gel, and the cell viability was checked. The manipulated cells show more than 90 % viability. The results indicate that the spheroids can be assembled inside thermoresponsive polymer solution to construct 3D in vitro cell systems with keeping their live conditions.
Keywords :
cellular biophysics; intelligent materials; microassembling; pattern formation; polymer gels; thermodynamic properties; tissue engineering; 3D cell structure construction; 3D in vitro cell system construction; cell spheroid manipulation; cell spheroid microassembly; cell viability checking; live cell condition; thermoresponsive gel probe; thermoresponsive polymer solution; tissue engineering application; Assembly; Fluorescence; In vitro; Polymers; Probes; Three-dimensional displays; Tissue engineering;
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2014 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4799-6678-3
DOI :
10.1109/MHS.2014.7006124
