Title :
Detection of influenza virus subtype using quartz crystal microbalance
Author :
Kagiyama, Shuichiro ; Masuda, T. ; Kaihatsu, Kunihiro ; Kato, Nei ; Arai, Fumihito
Author_Institution :
Grad. Sch. of Eng., Nagoya Univ., Nagoya, Japan
Abstract :
We fabricated a microfluidic chip to detect purified influenza virus RNA using Quartz Crystal Microbalance (QCM). The channel made of PDMS (poly dimethylsiloxane) was combined with QCM directly. The targeted virus genome introduced to the channel was captured by Peptide Nucleic Acid (PNA) immobilized on the electrode of QCM. After this, we introduced the gold colloid to be captured by influenza virus RNA to increase weight of virus RNA. By this, we could detect influenza virus RNA (106 pfu/mL).
Keywords :
RNA; bioMEMS; biosensors; colloids; genomics; gold; microelectrodes; microfluidics; microorganisms; microsensors; molecular biophysics; polymers; quartz crystal microbalances; Au; PDMS; electrode; gold colloid; immobilized peptide nucleic acid; influenza virus subtype detection; microfluidic chip; polydimethylsiloxane; purified influenza virus RNA; quartz crystal microbalance; targeted virus genome; virus RNA weight; Colloidal crystals; Educational institutions; Gold; Influenza; Microfluidics; Oscillators; RNA;
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2014 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4799-6678-3
DOI :
10.1109/MHS.2014.7006130
