Detection of influenza virus subtype using quartz crystal microbalance

Author

Kagiyama, Shuichiro ; Masuda, T. ; Kaihatsu, Kunihiro ; Kato, Nei ; Arai, Fumihito

Author_Institution

Grad. Sch. of Eng., Nagoya Univ., Nagoya, Japan

fYear

2014

fDate

10-12 Nov. 2014

Firstpage

1

Lastpage

2

Abstract

We fabricated a microfluidic chip to detect purified influenza virus RNA using Quartz Crystal Microbalance (QCM). The channel made of PDMS (poly dimethylsiloxane) was combined with QCM directly. The targeted virus genome introduced to the channel was captured by Peptide Nucleic Acid (PNA) immobilized on the electrode of QCM. After this, we introduced the gold colloid to be captured by influenza virus RNA to increase weight of virus RNA. By this, we could detect influenza virus RNA (10⁶ pfu/mL).

Keywords

RNA; bioMEMS; biosensors; colloids; genomics; gold; microelectrodes; microfluidics; microorganisms; microsensors; molecular biophysics; polymers; quartz crystal microbalances; Au; PDMS; electrode; gold colloid; immobilized peptide nucleic acid; influenza virus subtype detection; microfluidic chip; polydimethylsiloxane; purified influenza virus RNA; quartz crystal microbalance; targeted virus genome; virus RNA weight; Colloidal crystals; Educational institutions; Gold; Influenza; Microfluidics; Oscillators; RNA;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2014 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4799-6678-3

Type

conf

DOI

10.1109/MHS.2014.7006130

Filename

7006130