Real-time local environmental stimulation system with visual feedback control

Single cell analysis has attracted much attention for revealing detailed and localized biological information. A local environmental control technique is desired when analyzing the detailed and localized properties of single cells. In this paper, we propose a local environmental stimulation system which causes chemical stimulation in local area with micro dual-pipettes dynamically, freely, and automatically. As a cell analysis system, system quantification is necessary, so we try to realize it by using a fluorescent substance. Moreover, combining the spouted pipette and sucked pipette, we propose more localized stimulating system. As a first step, we try to measure the light intensity of the fluorescent substance using this system.