Title :
Quantitative image analyses of nuclear dynamics in migrating neurons
Author :
Umeshima, Hiroki ; Kure, Yuu ; Nomura, Keigo ; Yoshikawa, Shuhei ; Sakuma, Shinya ; Arai, Fumihito ; Kaneko, Makoto ; Kengaku, Mineko
Author_Institution :
Inst. for Integrated Cell-Mater. Sci. (WPI-iCeMS), Kyoto Univ., Kyoto, Japan
Abstract :
In this paper, we present a novel method to investigate the mechanism driving nuclear translocation in migrating neurons via digital image analyses of the nucleus. We obtained and quantitatively analyzed time-lapse images of the fluorescently labeled nucleus with a high-speed confocal microscope. We focused on spatiotemporal dynamics of the boundary curvature of the nucleus during nuclear translocation. Our data suggest major contribution of a pulling force to nuclear translocation during neuronal migration.
Keywords :
biomedical optical imaging; cellular transport; fluorescence; medical image processing; neurophysiology; optical microscopy; spatiotemporal phenomena; boundary curvature; digital image analyses; fluorescent-labeled nucleus; high-speed confocal microscopy; neuronal migration; nuclear dynamics; nuclear translocation; pulling force; quantitative image analyses; spatiotemporal dynamics; time-lapse images; Dynamics; Educational institutions; Force; Microscopy; Neurons; Proteins; Shape;
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2014 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4799-6678-3
DOI :
10.1109/MHS.2014.7006140
