Title :
Visualization of tissue collagen with femtosecond laser: application to skin diagnosis and cell culture
Author_Institution :
Dept. of Mech. Sci. & Eng., Osaka Univ., Toyonaka, Japan
Abstract :
Femtosecond laser induced second harmonic generation (SHG) microscopy provides non-invasive and non-staining observation of collagen structure. In this article, application of SHG microscopy for skin diagnosis and for engineered cell culture are demonstrated.
Keywords :
biomedical engineering; biomedical equipment; biomedical optical imaging; cellular biophysics; high-speed optical techniques; laser applications in medicine; molecular biophysics; molecular configurations; optical harmonic generation; optical microscopy; proteins; skin; SHG microscopy application; cell culture application; cell culture engineering; femtosecond laser; noninvasive collagen structure observation; nonstaining collagen structure observation; second harmonic generation microscopy; skin diagnosis application; tissue collagen visualization; Laser mode locking; Measurement by laser beam; Microscopy; Optical attenuators; Optical fiber polarization; Optical microscopy; Skin; SHG light; collagen; engineered cell; in situ visualization; laser microscope;
Conference_Titel :
CPMT Symposium Japan (ICSJ), 2014 IEEE
Conference_Location :
Kyoto
Print_ISBN :
978-1-4799-6194-8
DOI :
10.1109/ICSJ.2014.7009594
