Rapid Detection of E. coli Bacteria Using Potassium-Sensitive FETs in CMOS

Author

Nikkhoo, Nasim ; Gulak, P. Glenn ; Maxwell, Karen

Author_Institution

Dept. of Electr. & Comput. Eng., Univ. of Toronto, Toronto, ON, Canada

Volume

7

Issue

5

fYear

2013

fDate

Oct. 2013

Firstpage

621

Lastpage

630

Abstract

A novel integrated system for the detection of live bacteria in less than 10 minutes is presented. It utilizes the specificity of bacteriophages as biological detection elements with the sensitivity of integrated ion-selective field-effect transistors (ISFETs) implemented in conventional 0.18 μm CMOS with additional post-processes PVC-based potassium-sensitive membrane to provide a rapid, low-cost bacteria detection platform. Experimental methods to cancel ISFET non-idealities as well as data processing techniques to enhance detection capability of the bacteria sensor are demonstrated. Three groups of experimental results are provided using four strains of E. coli with two bacteriophages at two different temperatures. Measurements incorporating positive and negative control experiments are presented that successfully exhibit sensor specificity as well detection capability.

Keywords

CMOS integrated circuits; biomembranes; biosensors; cellular biophysics; ion sensitive field effect transistors; microorganisms; E. coli bacteria detection; bacteria sensor; bacteriophages; biological detection elements; conventional CMOS; data processing techniques; integrated ion-selective field-effect transistors; integrated system; live bacteria detection; negative control experiments; positive control experiments; post-process PVC-based potassium-sensitive membrane; potassium-sensitive FET; size 0.18 mum; Biomembranes; CMOS integrated circuits; DNA; Electrodes; Field effect transistors; Microorganisms; Bacteria detection; CMOS; E. coli; bacteriophage; biosensor; integrated circuits; ion-selective field-effect transistor (ISFET); potassium-selective membrane; Automatic Data Processing; Bacteriophages; Biosensing Techniques; Equipment Design; Escherichia coli; Potassium; Sensitivity and Specificity; Transistors, Electronic;

fLanguage

English

Journal_Title

Biomedical Circuits and Systems, IEEE Transactions on

Publisher

ieee

ISSN

1932-4545

Type

jour

DOI

10.1109/TBCAS.2013.2276013

Filename

6605563