A novel real-time immunoassay approach utilizing an electro-immunosensing microchip

Author

Li, Min ; Lin, Yu-Chew ; Ka-Chu Su ; Wang, Yu-Tsung ; Chang, Tsung Chain ; Lin, Hong-Ping

Author_Institution

Dept. of Eng. Sci., Nat. Cheng Kung Univ., Tainan, Taiwan

Volume

2

fYear

2005

fDate

5-9 June 2005

Firstpage

1620

Abstract

This study was focused on the development of an electro-immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme conjugated with secondary antibody for detection, the process of electro-immunoassay was relatively simpler in comparison to the conventional enzyme-linked immunosorbent assay (ELISA). Based on the impedance variety in the frequency domain, we could successfully identify 1 ng/mL protein A with nonspecific antigen as the negative control. The EIS chip had higher sensitivity and shorter assay time than the ELISA. Furthermore, the EIS chip could detect the kinematics of antibody-antigen binding.

Keywords

biological techniques; biosensors; enzymes; microsensors; molecular biophysics; EIS chip; antibody-antigen binding; antibody-antigen recognition; electro-immunosensing microchip; enzyme-linked immunosorbent assay; real-time immunoassay approach; Biochemistry; Electrodes; Frequency; Immune system; Impedance measurement; Kinematics; Laboratories; Proteins; Surface discharges; Transistors;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. The 13th International Conference on

Print_ISBN

0-7803-8994-8

Type

conf

DOI

10.1109/SENSOR.2005.1497398

Filename

1497398