Title :
An ultra-high sensitive protein lab-on-a-chip on polymer for prostate cancer diagnostics
Author :
Kai, Junhai ; Thati, Shilpa ; Ahn, Chong H.
Author_Institution :
Dept. of Electr. & Comput. Eng. & Comput. Sci., Cincinnati Univ., OH, USA
Abstract :
In this paper, we report a disposable, fast and high sensitive protein lab-on-a-chip on cyclic olefin copolymer (COC) substrate, which can be used for the detection of prostate specific antigen (PSA), a biomarker of prostate cancer. In this protein lab-on-a-chip, chemiluminescence-based sandwich immunoassay was developed for detecting PSA with the detection limit of 1 ng/mL. With the developed immunoassay, the protein lab-on-a-chips on polymer have successfully detected PSA in the range of 4-200 ng/mL. The protein lab-on-a-chip developed in this work can also be used for the detection of other biomarkers and proteins.
Keywords :
biochemistry; biological techniques; biosensors; cancer; chemiluminescence; molecular biophysics; patient diagnosis; polymer blends; chemiluminescence; cyclic olefin copolymer substrate; prostate cancer biomarker; prostate cancer diagnostics; prostate specific antigen detection; protein lab-on-a-chips; sandwich immunoassay; Biochemistry; Biomarkers; Blood; Diseases; Immune system; Lab-on-a-chip; Polymers; Prostate cancer; Protein engineering; Solids;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. The 13th International Conference on
Print_ISBN :
0-7803-8994-8
DOI :
10.1109/SENSOR.2005.1497406
