Temperature distribution measurement on micro-thermo device for single biomolecular observation using fluorescent dye

Author

Arata, Hidewki F. ; Bergaud, C. ; Noji, H. ; Takeuchi, S. ; Fujita, H.

Author_Institution

Inst. of Ind. Sci., Tokyo Univ., Japan

Volume

2

fYear

2005

fDate

5-9 June 2005

Firstpage

1672

Abstract

We have developed a new method to measure the temperature distribution in water with high spatial resolution. The temperature distribution was successfully calculated from the fluorescent intensity distribution of the Rhodamin B solution on the micro-thermo device for single molecular observation. With this method, the direct measurement of local temperature, where the molecule of interest stands, can be achieved with accuracy of 2 °C and 5.3 μm in spatial resolution.

Keywords

biochemistry; dyes; fluorescence; micromechanical devices; molecular biophysics; temperature distribution; temperature measurement; 2 C; Fl-ATPase; Rhodamin B solution; fluorescent dye; fluorescent intensity distribution; microthermo device; single biomolecular observation; temperature distribution measurement; Cameras; Fluorescence; Glass; Proteins; Spatial resolution; Temperature dependence; Temperature distribution; Temperature measurement; Temperature sensors; Water heating;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. The 13th International Conference on

Print_ISBN

0-7803-8994-8

Type

conf

DOI

10.1109/SENSOR.2005.1497411

Filename

1497411