Title :
Temperature distribution measurement on micro-thermo device for single biomolecular observation using fluorescent dye
Author :
Arata, Hidewki F. ; Bergaud, C. ; Noji, H. ; Takeuchi, S. ; Fujita, H.
Author_Institution :
Inst. of Ind. Sci., Tokyo Univ., Japan
Abstract :
We have developed a new method to measure the temperature distribution in water with high spatial resolution. The temperature distribution was successfully calculated from the fluorescent intensity distribution of the Rhodamin B solution on the micro-thermo device for single molecular observation. With this method, the direct measurement of local temperature, where the molecule of interest stands, can be achieved with accuracy of 2 °C and 5.3 μm in spatial resolution.
Keywords :
biochemistry; dyes; fluorescence; micromechanical devices; molecular biophysics; temperature distribution; temperature measurement; 2 C; Fl-ATPase; Rhodamin B solution; fluorescent dye; fluorescent intensity distribution; microthermo device; single biomolecular observation; temperature distribution measurement; Cameras; Fluorescence; Glass; Proteins; Spatial resolution; Temperature dependence; Temperature distribution; Temperature measurement; Temperature sensors; Water heating;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. The 13th International Conference on
Print_ISBN :
0-7803-8994-8
DOI :
10.1109/SENSOR.2005.1497411
