Identifying proteins for microbiosensor applications

Experiments with two classes of protein, namely, nutrient transport proteins of the gram-negative bacteria and monoclonal antibodies, are reported. Initial experiments indicate that immobilization of antifluorescein antibodies causes a decrease in affinity. The decrease in affinity appears to be antibody-dependent. Experiments with a phosphate transport system protein indicate that the immobilization chemistry is important in designing sensors with low-background, nonspecific adsorption. Experiments with the phosphate transport protein also indicate that it will be possible to fabricate bioreactors for the continuous production of proteins that are useful for microbiosensor applications