Dry-Reagent Storage for Disposable Lab-On-Card Diagnosis of Enteric Pathogens

Author

Ramachandran, S. ; Gerdes, J. ; Tarr, P. ; Yager, P. ; Dillman, L. ; Peck, R. ; Kokoris, M. ; Nabavi, M. ; Battrell, F. ; Hoekstra, D. ; Weigl, B.H.

Author_Institution

Dept. of Bioeng., Washington Univ., Seattle, WA

fYear

2006

fDate

2-4 April 2006

Firstpage

16

Lastpage

19

Abstract

This paper presents a process for dry-reagent storage and handling for a microfluidic lab-on-a-card platform for point-of-care applications to identify enteric bacterial pathogens in patients presenting with acute diarrhea, with special reference to infections that might be encountered in developing countries. Three different reagents are studied: i) antibody-coated magnetic beads for pathogen capture, ii) lysis buffer for extraction of nucleic acids from the pathogens, and iii) DNA amplification of the nucleic acids by polymerase chain reaction. This presentation will report on the process of dry reagent storage, resuspension and its functionality

Keywords

DNA; biochemistry; biosensors; cellular biophysics; diseases; microfluidics; microorganisms; microsensors; molecular biophysics; patient diagnosis; proteins; DNA amplification; acute diarrhea; antibody-coated magnetic beads; disposable microfluidic lab-on-a-card diagnosis; dry-reagent handling; dry-reagent storage; enteric bacterial pathogens; lysis buffer; nucleic acid extraction; polymerase chain reaction; Contamination; DNA; Diseases; Immune system; Medical services; Microfluidics; Microorganisms; Pathogens; Polymers; Water pollution;

fLanguage

English

Publisher

ieee

Conference_Titel

Distributed Diagnosis and Home Healthcare, 2006. D2H2. 1st Transdisciplinary Conference on

Conference_Location

Arlington, VA

Print_ISBN

1-4244-0058-9

Type

conf

DOI

10.1109/DDHH.2006.1624786

Filename

1624786