Separation of cephalosporins using ionic and neutral polimeric resins

In this paper, cephamycin C purification using column adsorption processes was investigated. Two polymeric resins were used, Q-Sepharose XL, an ionic resin, and Amberlite XAD-4, a neutral one. The column process using QXL separated two fractions with antibacterial activity. Cephamycin C was present only in the first fraction, called F1, but was not pure. In the column process using the resin XAD-4, it was not observed any separation of different compounds when the feed was the ultrafiltered broth. Nevertheless, the system was able to separate two fractions when the feed was the fraction F1. When cephalosporin C was added to F1, the column with XAD-4 also separated cephamycin C from cephalosporin C.