Quantitative FTIR spectrophotometry of cholesterol and other blood constituents and their interference with the in-vitro measurement of blood glucose

Author

Stohr, Erich ; Bhandare, Prashant ; Peura, Robert A. ; Mendelson, Yitzhak

Author_Institution

Dept. of Biomed. Eng., Worcester Polytech. Inst., MA, USA

fYear

1992

fDate

1992

Firstpage

105

Lastpage

106

Abstract

Albumin, ethanol, and urea were measured in-vitro using Fourier transform infrared (FTIR) spectroscopy. The peak height and corresponding peak area of the spectra were computed for concentration prediction. This univariate method was compared with partial least squares (PLS) and principal component regression (PCR) multivariate routines. The standard errors of calibration obtained were cholesterol (7.8 mg/dl), albumin (49.1 mg/dl), ethanol (7.8 mg/dl), lactic acid (3.3 mg/dl), and urea (8.4 mg/dl). Amino acids, uric acid, glycerol, and glycogen were studied to determine their interference with good glucose measurements.

Keywords

Fourier transform spectroscopy; biomedical measurement; biosensors; blood; organic compounds; spectrochemical analysis; spectrophotometry; statistical analysis; albumin; amino acids; blood constituents; blood glucose; calibration errors; cholesterol; concentration prediction; ethanol; glycerol; glycogen; in-vitro measurement; lactic acid; multivariate routines; partial least squares; peak area; peak height; principal component regression; quantitative FTIR spectrophotometry; univariate method; urea; uric acid; Blood; Calibration; Ethanol; Fourier transforms; In vitro; Infrared spectra; Interference; Least squares methods; Spectroscopy; Sugar;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference, 1992., Proceedings of the 1992 Eighteenth IEEE Annual Northeast

Conference_Location

Kingston, RI, USA

Print_ISBN

0-7803-0902-2

Type

conf

DOI

10.1109/NEBC.1992.285974

Filename

285974