Title :
Wide-field extrinsic optical signal imaging of fluorescence potassium sensors
Author :
Pujari, R.A. ; Shitole, P.S. ; Charnani, V.S. ; McShane, M.J. ; Robinson, C.J.
Author_Institution :
Biomedical Eng. Program, Louisiana State Univ., Ruston, LA
Abstract :
Wide-field extrinsic optical signal (EOS) imaging is an excellent method for its spatial resolution of 50-100 mum and a wide imaging field of several mm that cannot be achieved by any microscope. Fluorescence images of a nanoengineered sensor for potassium, fabricated by using electrostatic layer-by-layer (LbL) self-assembly technique, were collected by a wide-field EOS imaging system under different concentrations of [K+]. The images were analyzed using MATLAB to study the quenching properties of the potassium nanosensor
Keywords :
bioelectric phenomena; biomedical optical imaging; electric sensing devices; fluorescence; microsensors; nanotechnology; optical sensors; potassium; self-assembly; K; MATLAB; electrostatic layer-by-layer self-assembly; fluorescence images; fluorescence potassium sensors; nanoengineered sensor; quenching properties; wide-field extrinsic optical signal imaging; Earth Observing System; Electrostatics; Fluorescence; Image sensors; Optical imaging; Optical microscopy; Optical sensors; Self-assembly; Sensor systems; Spatial resolution;
Conference_Titel :
Biomedical Imaging: Nano to Macro, 2006. 3rd IEEE International Symposium on
Conference_Location :
Arlington, VA
Print_ISBN :
0-7803-9576-X
DOI :
10.1109/ISBI.2006.1625033
