• DocumentCode
    1818210
  • Title

    Backprojection-based reconstruction and correction for distance-dependent defocus in cryoelectron microscopy

  • Author

    Kazantsev, Ivan G. ; Herman, Gabor T. ; Cernetic, Laslo

  • Author_Institution
    Mater. Sci. Dept., DTU, Roskilde
  • fYear
    2008
  • fDate
    14-17 May 2008
  • Firstpage
    133
  • Lastpage
    136
  • Abstract
    We study two methods of micrograph processing in cryo- electron microscopy: the defocus-gradient corrected back- projection algorithm and the correction of micrographs for the contrast transfer function based on the frequency-distance relation. Analyzing integral images produced by the methods we conclude that they are asymptotically equivalent within the framework of stationary phase approximation.
  • Keywords
    electron microscopy; image reconstruction; medical image processing; optical focusing; optical transfer function; backprojection; contrast transfer function; cryoelectron microscopy; distance-dependent defocus; image correction; image reconstruction; micrograph processing; Computed tomography; Computer graphics; Computer science; Electron microscopy; Frequency; Image analysis; Image reconstruction; Materials science and technology; Transfer functions; Transmission electron microscopy; CTF correction; Electron microscopy;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Biomedical Imaging: From Nano to Macro, 2008. ISBI 2008. 5th IEEE International Symposium on
  • Conference_Location
    Paris
  • Print_ISBN
    978-1-4244-2002-5
  • Electronic_ISBN
    978-1-4244-2003-2
  • Type

    conf

  • DOI
    10.1109/ISBI.2008.4540950
  • Filename
    4540950