Title :
Backprojection-based reconstruction and correction for distance-dependent defocus in cryoelectron microscopy
Author :
Kazantsev, Ivan G. ; Herman, Gabor T. ; Cernetic, Laslo
Author_Institution :
Mater. Sci. Dept., DTU, Roskilde
Abstract :
We study two methods of micrograph processing in cryo- electron microscopy: the defocus-gradient corrected back- projection algorithm and the correction of micrographs for the contrast transfer function based on the frequency-distance relation. Analyzing integral images produced by the methods we conclude that they are asymptotically equivalent within the framework of stationary phase approximation.
Keywords :
electron microscopy; image reconstruction; medical image processing; optical focusing; optical transfer function; backprojection; contrast transfer function; cryoelectron microscopy; distance-dependent defocus; image correction; image reconstruction; micrograph processing; Computed tomography; Computer graphics; Computer science; Electron microscopy; Frequency; Image analysis; Image reconstruction; Materials science and technology; Transfer functions; Transmission electron microscopy; CTF correction; Electron microscopy;
Conference_Titel :
Biomedical Imaging: From Nano to Macro, 2008. ISBI 2008. 5th IEEE International Symposium on
Conference_Location :
Paris
Print_ISBN :
978-1-4244-2002-5
Electronic_ISBN :
978-1-4244-2003-2
DOI :
10.1109/ISBI.2008.4540950
