Mixed-monolayers with alkane thiol on gold as substrates for microarray applications

Author

Mihailescu, Carmen-Marinela ; Stan, Dana ; Ruta, Lavinia ; Ion, Baciu ; Moldovan, Carmen ; Schiopu, Vasilica ; Simion, Monica ; Gavrila, Raluca

Author_Institution

Fac. of Chem, Univ. of Bucharest, Bucharest

Volume

1

fYear

2008

fDate

13-15 Oct. 2008

Firstpage

173

Lastpage

176

Abstract

In this work we report a self-assembled mixt into plat silicon (Si n-type, 10-20 Omegacm) coated with gold deposited by evaporation. The formation of mixed self-assembly monolayers (SAM) was characterized with FTIR-ATR, and cyclic voltammetry techniques. In order to assay the functionality of SAMs prepared for immobilization of high density of functional protein we used different concentrations of BSA in PBS. The protein (BSA) was labeled with Cy3 dye and printed onto coated slides using a microarray printer. The intensity of signal detected, for any one spot, is proportional to the quantity of antigen (BSA) bound on the surface. Those are preliminary experiments in order to test this surface for to immobilize one of the most pathogens Escherichia Coli O157:H7.

Keywords

Fourier transform spectra; attenuated total reflection; biosensors; gold; infrared spectra; microorganisms; monolayers; proteins; proteomics; self-assembly; silicon; voltammetry (chemical analysis); Au; BSA protein; Cy3 dye labeling; Escherichia coli; FTIR-ATR analysis; PBS; Si; alkane thiol-on-gold substrates; bovine serum albumin; cyclic voltammetry; functional protein immobilization; gold coated plat silicon; microarray applications; microarray printer; mixed SAM; self assembled mixed monolayers; signal intensity; Chemistry; Gold; Immune system; Microscopy; Pathogens; Printers; Protein engineering; Silicon; Surface topography; Testing; BSA; E.coli; SAMs; gold; microarray;

fLanguage

English

Publisher

ieee

Conference_Titel

Semiconductor Conference, 2008. CAS 2008. International

Conference_Location

Sinaia

ISSN

1545-827X

Print_ISBN

978-1-4244-2004-9

Type

conf

DOI

10.1109/SMICND.2008.4703361

Filename

4703361