Towards high-throughput FLIM for protein-protein interaction screening of live cells and tissue microarrays

Author

Barber, P.R. ; Pierce, G.P. ; Ameer-Beg, S.M. ; Matthews, D.R. ; Carlin, L.M. ; Keppler, M. ; Kelleher, M. ; Festy, F. ; Gillett, C. ; Springall, R. ; Ng, T.C. ; Vojnovic, B.

Author_Institution

Gray Cancer Inst., Oxford Univ., Northwood

fYear

2008

fDate

14-17 May 2008

Firstpage

356

Lastpage

359

Abstract

Studying cellular protein-protein interactions in situ requires a technique such as fluorescence resonance energy transfer (FRET) which is sensitive on the nanometer scale. Observing FRET is significantly simplified if the fluorescence lifetime of the donor can be monitored. Results from live cells and tissue micro arrays are presented from an automated microscope incorporating time-domain TCSPC fluorescence lifetime imaging (FLIM). Novel hardware and software with a modular approach and scripting abilities allow us to work towards speed-optimized acquisition and ease of use to bring FLIM into the high-throughput regime.

Keywords

biological techniques; biological tissues; cellular biophysics; fluorescence; molecular biophysics; proteins; TCSPC FLIM; fluorescence lifetime imaging; fluorescence resonance energy transfer; live cells; protein-protein interaction; tissue microarrays; Automatic control; Biological tissues; Cancer detection; Educational institutions; Energy resolution; Fluorescence; Hardware; Optical microscopy; Proteins; Time domain analysis; FRET/FLIM; High-Content Screening; Protein-protein interactions; Tissue Micro Arrays;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging: From Nano to Macro, 2008. ISBI 2008. 5th IEEE International Symposium on

Conference_Location

Paris

Print_ISBN

978-1-4244-2002-5

Electronic_ISBN

978-1-4244-2003-2

Type

conf

DOI

10.1109/ISBI.2008.4541006

Filename

4541006