• DocumentCode
    1826756
  • Title

    Detection of acid phosphatase activity using ATP conferred protection of AgNPs against salt induced aggregation

  • Author

    Tagad, Chandrakant K. ; Sabharwal, Sushma G. ; Aiyer, R.C.

  • Author_Institution
    Dept. of Chemstry, Savitribai Phule Pune Univ., Pune, India
  • fYear
    2015
  • fDate
    7-10 March 2015
  • Firstpage
    173
  • Lastpage
    176
  • Abstract
    ATP conferred protection of silver nanoparticles (AgNPs) against salt induced aggregation has been used as a simple, sensitive and cost effective tool for detection of acid phosphatase (ACP) activity. In the absence of the enzymes, unreacted ATP protects AgNPs from salt-induced aggregation. Dephosphorylation of ATP in the presence of the enzyme reduces the protecting action of ATP and thus there is aggregation of AgNPs. Developed sensing tool for the detection of ACP were found to be suitable in the range of 0.6 to 7 units per ml.
  • Keywords
    aggregation; biochemistry; enzymes; molecular biophysics; nanomedicine; nanoparticles; silver; sodium compounds; ATP conferred protection; ATP dephosphorylation; AgNO3; NaCl; acid phosphatase activity detection; salt induced aggregation; silver nanoparticles; Absorption; Gold; Nanoparticles; Proteins; Sensors; Silver; AgNPs; Optical detection; acid phosphatase;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Physics and Technology of Sensors (ISPTS), 2015 2nd International Symposium on
  • Conference_Location
    Pune
  • Type

    conf

  • DOI
    10.1109/ISPTS.2015.7220106
  • Filename
    7220106
    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=1826756