Title :
Cells tracking in a live zebrafish embryo
Author :
Melani, C. ; Peyrieras, N. ; Mikula, K. ; Zanella, C. ; Campana, M. ; Rizzi, B. ; Veronesi, F. ; Sarti, A. ; Lombardot, B. ; Bourgine, P.
Author_Institution :
Univ. di Bologna, Bologna
Abstract :
We designed a set of procedures for achieving the tracking of cell nuclei and the identification of cell divisions in live zebrafish embryos using 3D+time images acquired by confocal laser scanning microscopy (CLSM). Our strategy includes image signal enhancement with feature preserving denoising algorithm, automated identification of the nuclei position, extraction of the optical flow from 3D images sequences and tracking of nuclei.
Keywords :
biomedical optical imaging; cellular biophysics; image denoising; image enhancement; image sequences; laser applications in medicine; medical image processing; optical microscopy; optical tracking; 3D images sequences; 3D-time images; CLSM; automated identification; cell division identification; cell nuclei position; cell nuclei tracking; confocal laser scanning microscopy; feature preserving denoising algorithm; image signal enhancement; live zebrafish embryo; optical flow extraction; Biomembranes; Embryo; Image reconstruction; In vivo; Microscopy; Noise reduction; Optical design; Optical imaging; Proteins; Signal processing; Algorithms; Animals; Artificial Intelligence; Embryo, Nonmammalian; Image Enhancement; Image Interpretation, Computer-Assisted; Imaging, Three-Dimensional; Microscopy, Confocal; Microscopy, Fluorescence; Pattern Recognition, Automated; Reproducibility of Results; Sensitivity and Specificity; Zebrafish;
Conference_Titel :
Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE
Conference_Location :
Lyon
Print_ISBN :
978-1-4244-0787-3
DOI :
10.1109/IEMBS.2007.4352619
