• DocumentCode
    1829523
  • Title

    Sensors on surfaces

  • Author

    Schoen, P.E. ; Fare, T. ; Bhatia, S.K. ; Shriver-Lake, L.C. ; Ligler, F.S.

  • Author_Institution
    US Naval Res. Lab., Washington, DC, USA
  • fYear
    1989
  • fDate
    9-12 Nov 1989
  • Firstpage
    1379
  • Abstract
    Immobilization of the enzyme acetylcholinesterase on a glass substrate has been demonstrated. The substrate was prepared with a thiol-terminal silane, to which was bonded the heterobifunctional cross-linker, N-gamma-maleimidobutyryloxy succinimide ester. The terminal amino groups of the enzyme were then attached to the cross-linker, yielding a surface enzyme coverage of 0.46 ng/mm2 with approximately 40% of the activity of the bound enzymes retained. Strategies for fabrication of practical biosensors are discussed
  • Keywords
    biosensors; proteins; N-gamma-maleimidobutyryloxy succinimide ester; acetylcholinesterase; biosensors fabrication; glass substrate; heterobifunctional cross-linker; immobilized enzyme; surface enzyme coverage; thiol-terminal silane; Biochemical analysis; Biochemistry; Biosensors; Bonding; Detectors; Differential amplifiers; Electrodes; Glass; Proteins; Substrates;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Engineering in Medicine and Biology Society, 1989. Images of the Twenty-First Century., Proceedings of the Annual International Conference of the IEEE Engineering in
  • Conference_Location
    Seattle, WA
  • Type

    conf

  • DOI
    10.1109/IEMBS.1989.96250
  • Filename
    96250
    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=1829523