A handheld fluorometer for measuring cellular metabolism

Author

Nelson, Nicole ; Sander, David ; Dandin, Marc ; Sarje, Anshu ; Prakash, Somashekar ; Ji, Honghao ; Abshire, Pamela

Author_Institution

Dept. of Electr. & Comput. Eng., Univ. of Maryland, College Park, MD

fYear

2008

fDate

18-21 May 2008

Firstpage

1080

Lastpage

1083

Abstract

We demonstrate the application of a handheld fluorometer optimized for UV excitable assays. We demonstrate the measurement of metabolic products as yeast cells germinate in dextrose solution. In particular we measure NADH which is produced during cellular respiration. The handheld fluorometer consists of a CMOS active pixel sensor with in-pixel CDS, coupled with a custom chromophore-polymer emission Alter and a UV LED as the excitation source. The handheld fluorometer is able to detect as little as 10 muM of NADH, and in its present format should be applicable to any fluorescence assay with UV excitation and visible emission wavelengths.

Keywords

CMOS image sensors; biomolecular electronics; CMOS active pixel sensor; UV LED; UV excitable assays; cellular metabolism; cellular respiration; chromophore-polymer emission Alter; emission wavelengths; handheld fluorometer; polymer emission Alter; Biochemistry; Detectors; Electromagnetic interference; Fluorescence; Fungi; Light emitting diodes; Microfluidics; Noise generators; Optical filters; Working environment noise;

fLanguage

English

Publisher

ieee

Conference_Titel

Circuits and Systems, 2008. ISCAS 2008. IEEE International Symposium on

Conference_Location

Seattle, WA

Print_ISBN

978-1-4244-1683-7

Electronic_ISBN

978-1-4244-1684-4

Type

conf

DOI

10.1109/ISCAS.2008.4541609

Filename

4541609