Implementation of Electrochemiluminescence Microanalysis in PCB Technology

Author

Pittet, P. ; Guo-Neng Lu ; Galvan, J.-M. ; Ferrigno, R. ; Stephan, K. ; Blum, L.J. ; Leca-Bouvier, B.D.

Author_Institution

Inst. des Nanotechnol. de Lyon, Villeurbanne

fYear

2007

fDate

22-26 Aug. 2007

Firstpage

2944

Lastpage

2947

Abstract

We present an instrumental development to implement electrochemiluminescence (ECL) microanalysis using printed circuit board (PCB) technology. PCB gold macro- (10 mm²) and micro- (0.09 mm²) electrodes and two ECL microfluidic devices are designed, fabricated and tested via luminol ECL detection. Potential modulation is performed between 0.7 and 0 V vs. Ag/AgCl for luminol oxidation, thus giving rise to on/off ECL responses in the presence of hydrogen peroxide. Synchronous detection is adopted to allow weak ECL signal recovery at a very low signal-to-noise ratio (SNR). The detection limit obtained with the two ECL microfluidic devices is 50 nM and 100 nM H₂O₂ for macroelectrodes and microelectrodes, respectively.

Keywords

biochemistry; biomedical electrodes; chemical analysis; chemiluminescence; electroluminescence; microelectrodes; microfluidics; printed circuits; ECL microfluidic devices; PCB gold electrodes; PCB technology; electrochemiluminescence microanalysis; luminol ECL detection; luminol oxidation; macroelectrodes; microelectrodes; potential modulation; printed circuit board; weak ECL signal recovery; Chemical technology; Copper; Electrodes; Fabrication; Gold; Instruments; Interference; Microfluidics; Printed circuits; Substrates; Electrochemistry; Electronics; Hydrogen Peroxide; Luminescent Agents; Luminescent Measurements; Luminol; Microelectrodes; Microfluidic Analytical Techniques; Silver; Silver Compounds;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE

Conference_Location

Lyon

ISSN

1557-170X

Print_ISBN

978-1-4244-0787-3

Type

conf

DOI

10.1109/IEMBS.2007.4352946

Filename

4352946