Continuous monitoring of histamine release from single KU-812 cell with an electrochemical cellular chip

In this study, we developed an electrochemical cellular chip for continuous monitoring of histamine release at single cell level. The cells were individually accommodated and stabilized inside a microwell electrode (MWE), and then the released histamine was electrochemically detected by the MWE. We employed KU-812 cells (ECACC no. 90071807) as a cell model, and a spiked amperogram was observed in accordance to the exocytotic event of histamine release. In our experiment, the investigated cells were stimulated with high K⁺ solution which increases a cytosolic Ca²⁺ influx. The amperograms (+0.6 V vs. Ag/AgCl) of single KU-812 cell with and without stimulation were compared, and an amperogram with higher spiked current were found in high K⁺ solution. Our result shows that a feasible application for the cell secretion measurement by using this device.