Development of single cell biochip platform based on silicon for multiple patch clamping technology

The single cell study is crucial for tissue engineering, drug screening, and the opening of DNA code for life expressions. To study the single cell, it is involved in the second messengers or the ion channel activities concerned with the electrophysiological phenomena. However, it is so difficult to get the ion channel activity by whole cell clamp or even patch clamp. How to release the difficulty in patching clamp for the world wide use in any lab as easy as PCR becomes a hot issue now. Biochip fabricated by N/MEMS technique is one alternative to make it come true and this is the solution in this paper to present the possible idea and workout. Here we present the cell attachment technique to grab the cell successfully and the N/MEMS process to make a micrometer hole in one of an array of electrode for the cell clamp. The breakthrough is coming soon to push the opening of DNA code theory forward with a great step.