Title :
An implantable green fluorescence imaging device using absorption filters with high excitation light rejection ratio
Author :
Sunaga, Yoshinori ; Haruta, Makito ; Yamaguchi, Toru ; Motoyama, Mayumi ; Ohta, Yoshichika ; Takehara, Hironari ; Noda, Toshio ; Sasagawa, Kiyotaka ; Tokuda, Takashi ; Ohta, Jun
Author_Institution :
Grad. Sch. of Mater. Sci., Nara Inst. of Sci. & Technol., Ikoma, Japan
Abstract :
We have developed an implantable complementary-metal-oxide-semiconductor (CMOS) imaging device for green fluorescence imaging to observe various neural activities of the mouse brain in a freely moving state. The device comprises a CMOS image sensor, blue LEDs as excitation light sources, and absorption filters to enable real-time green fluorescence imaging. To observe weak green fluorescence reactions such as that of green fluorescent protein (GFP), we achieved efficient excitation light rejection using a combination of dedicated absorption filters and achieved the detection of GFP positive cells from mouse brain slices. It is expected that high-sensitivity green fluorescence imaging of neural activities in a freely moving mouse will be possible using this technology.
Keywords :
CMOS image sensors; biomedical optical imaging; brain; cellular biophysics; fluorescence; light emitting diodes; light sources; GFP positive cell detection; absorption filters; blue LED; complementary-metal-oxide-semiconductor; excitation light sources; freely moving state; green fluorescent protein; high excitation light rejection ratio; implantable CMOS image sensor; implantable green fluorescence imaging device; mouse brain slices; neural activities; Absorption; CMOS integrated circuits; Fluorescence; Imaging; Light emitting diodes; Mice; Optical filters; CMOS implatable device; GFP; brain functional imaging; fluorescence imaging; freely moving;
Conference_Titel :
Biomedical Circuits and Systems Conference (BioCAS), 2014 IEEE
Conference_Location :
Lausanne
DOI :
10.1109/BioCAS.2014.6981709
