Unidirectional cellular durotaxis via microfabricated posts of varying anisotropy

Author

Sochol, Ryan D. ; Higa, Adrienne T. ; Janairo, Randall R R ; Chou, Annie ; Li, Song ; Lin, Liwei

Author_Institution

Berkeley Sensor & Actuator Center, Univ. of California at Berkeley, Berkeley, CA, USA

fYear

2009

fDate

21-25 June 2009

Firstpage

604

Lastpage

607

Abstract

This paper presents unprecedented accomplishments toward unidirectional guidance of cellular migration via durotaxis-based microtopography. In contrast to previous efforts, micropost arrays of varying anisotropy (muPVAs) optimize unidirectional control of cell migration through dual axis durotaxis cues which restrict movement in the lateral direction in addition to promoting migration in the direction of increasing micropost stiffness. Preliminary results show 79% of bovine aortic endothelial cells (BAECs) cultured on muPVA substrates migrated within plusmn60deg of the direction of increasing micropost anisotropy. muPVAs offer a simple, yet powerful technique for enabling unidirectional control of cellular migration for a variety of applications in tissue engineering, biomaterials, and medical device implantation.

Keywords

biomedical materials; cellular transport; prosthetics; tissue engineering; biomaterials; bovine aortic endothelial cells; cellular migration; dual axis durotaxis cues; durotaxis-based microtopography; medical device implantation; micropost anisotropy; tissue engineering; unidirectional cellular durotaxis; Actuators; Anisotropic magnetoresistance; Biological processes; Bovine; Cells (biology); Electrochemical machining; Fabrication; Resists; Tissue engineering; Wounds; Cell Migration; Durotaxis; Mechanotaxis; Micropillars; Microposts; Microtopography;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285397

Filename

5285397