Title :
High-throughput on-chip flow cytometry system using “microfluidic drifting” based three-dimensional (3D) hydrodynamic focusing
Author :
Mao, X. ; Lin, S.-C.S. ; Huang, T.J.
Abstract :
In this work we demonstrate the application of a three-dimensional (3D) hydrodynamic focusing technique, ldquomicrofluidic driftingrdquo in the development of a miniaturized on-chip flow cytometry system. ldquoMicrofluidic driftingrdquo utilizes viscous drag of Dean flow induced in a curved microfluidic channel to realize 3D hydrodynamic focusing in a single layer planar microfluidic device. Through force scaling analysis, numerical study, and experimental characterization, we show this technique can be successfully applied to focus large microparticles such as biological cells. A laser-induced fluorescence (LIF) detection system was incorporated with the 3D focusing device and a high-throughput (1700 cells/s) cell detection was demonstrated.
Keywords :
bioMEMS; biomedical measurement; cellular biophysics; drag; fluorescence; hydrodynamics; lab-on-a-chip; laser applications in medicine; microchannel flow; optical focusing; Dean flow; LIF detection system; biological cells; curved microfluidic channel; high-throughput on-chip flow cytometry system; laser-induced fluorescence; microfluidic drifting; single layer planar microfluidic device; three-dimensional hydrodynamic focusing; viscous drag; Biological cells; Biomedical engineering; Drag; Fluid flow measurement; Fluorescence; Force measurement; Hydrodynamics; Microfluidics; System-on-a-chip; Time measurement; 3D Hydrodynamic Focusing; Dean Flow; Flow Cytometry; Microfluidics;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285473
