Title :
A patch-clamp device with cell sorting and positioning functions
Author :
Penmetsa, Siva ; Nagrajan, Krithika ; Gong, Zhongcheng ; Mills, David ; Que, Long
Author_Institution :
Inst. for Micromanufacturing, Louisiana Tech Univ., Ruston, LA, USA
Abstract :
A patch-clamp (PC) microchip with cell sorting and positioning functions is reported, showing promise for an automated PC microsystem, which can potentially help avoid drawbacks of random cell selection or positioning for a PC microchip. The cell sorting and positioning are enabled by air bubble (AB) actuators. AB actuators are pneumatic actuators, in which air pressure is generated by microheaters within sealed microchambers. The sorting, positioning and capturing of 3T3 cells with a prototype device have been demonstrated successfully. Measurements found that the electrode impedance is 1.8 MOmega; the average seal resistance is 22 MOmega with a captured cell.
Keywords :
bioMEMS; biological techniques; bubbles; cellular biophysics; lab-on-a-chip; pneumatic actuators; 3T3 cells; air bubble actuators; cell positioning; cell sorting; microheaters; patch-clamp microchip; pneumatic actuators; Cells (biology); Electrodes; Glass; Immune system; Pneumatic actuators; Position measurement; Proteins; Prototypes; Semiconductor device measurement; Sorting; Automated patch-clamp device; air bubble actuators; cell positioning; cell sorting;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285558
