DocumentCode :
1855748
Title :
Blind Spectral Unmixing of M-FISH Images by Non-negative Matrix Factorization
Author :
Munoz-Barrutia, Arrate ; Garcia-Munoz, J. ; Ucar, B. ; Fernandez-Garcia, I. ; Ortiz-de-Solorzano, Carlos
Author_Institution :
Univ. of Navarra, Pamplona
fYear :
2007
fDate :
22-26 Aug. 2007
Firstpage :
6247
Lastpage :
6250
Abstract :
Multi-color fluorescent in-situ hybridization (M- FISH) selectively stains multiple DNA sequences using fluorescently labeled DNA probes. Proper interpretation of M-FISH images is often hampered by spectral overlap between the detected emissions of the fluorochromes. When using more than two or three fluorochromes, the appropriate combination of wide-band excitation and emission filters reduces cross-talk, but cannot completely eliminate it. A number of approaches -both hardware and software- have been proposed in the last decade to facilitate the interpretation of M-FISH images. The most used and efficient approaches use linear unmixing methods that algorithmically compute and correct for the fluorochrome contributions to each detection channel. In contrast to standard methods that require prior knowledge of the fluorochrome spectra, we present a new method, Non-Negative Matrix Factorization (NMF), that blindly estimates the spectral contributions and corrects for the overlap. Our experimental results show that its performance in terms of residual cross-talk and spot counting reliability outperforms the non-blind state-of-the-art method, the Non-Negative Least Squares (NNLS) algorithm.
Keywords :
DNA; biological techniques; biology computing; blind source separation; filtering theory; fluorescence; image colour analysis; matrix decomposition; molecular biophysics; blind spectral unmixing method; cross-talk reduction; emission filters; fluorescently labeled DNA probes; fluorochromes emission; multicolor fluorescent in-situ hybridization images; multiple DNA sequences; nonnegative matrix factorization; residual cross-talk terms; spot counting reliability; wide-band excitation filters; DNA; Filters; Fluorescence; Hardware; Least squares methods; Marine animals; Matrix decomposition; Probes; Sequences; Wideband; Algorithms; Artificial Intelligence; Image Enhancement; Image Interpretation, Computer-Assisted; In Situ Hybridization, Fluorescence; Microscopy, Fluorescence; Pattern Recognition, Automated; Reproducibility of Results; Sensitivity and Specificity;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE
Conference_Location :
Lyon
ISSN :
1557-170X
Print_ISBN :
978-1-4244-0787-3
Type :
conf
DOI :
10.1109/IEMBS.2007.4353783
Filename :
4353783
Link To Document :
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