DocumentCode :
1858923
Title :
Fabrication and self-assembly of movable microstructures embedding cells with concentration control inside microfluidic devices
Author :
Tao Yue ; Nakajima, Masahiro ; Yajing Shen ; Tajima, Hirotaka ; Fukuda, Toshio
Author_Institution :
Dept. of Micro-Nano Syst. Eng., Nagoya Univ., Nagoya, Japan
fYear :
2012
fDate :
4-7 Nov. 2012
Firstpage :
169
Lastpage :
174
Abstract :
Currently the research about large quantities cells assembly is seriously regarded, since it can provide high efficiency methods for artificial tissue engineering. In this paper, we report a novel cell assembly method based on cell immobilization by photo-crosslinkable resin, cell manipulation by dielectrophoresis (DEP) and microfluidic self-assembly inside microfluidic devices. The on-chip fabrication of movable microstructures embedding yeast cells (W303) based on Poly (ethylene glycol) Diacrylate (PEGDA) was reported. Novel microelectrodes are fabricated by Cr/Au for manipulating cells before immobilization. A microfluidic chip for controlling cell concentration with separated patterning and fabrication areas is fabricated. Movable microstructures embedding microbeads of which the concentration is controllable are fabricated in the microfluidic channel, inside PEGDA and NaCl solution. A 2-layer microfluidic device is fabricated by Polydimethylsiloxane (PDMS) and self-assembly method of the fabricated movable microstructures via this device is preliminarily demonstrated.
Keywords :
bioMEMS; biological techniques; cellular biophysics; electrophoresis; lab-on-a-chip; microelectrodes; microfabrication; microfluidics; polymers; self-assembly; NaCl; W303; artificial tissue engineering; cell assembly method; cell concentration; cell immobilization; cell manipulation; dielectrophoresis; microbeads; microelectrodes; microfluidic channel; microfluidic chip; microfluidic devices; microfluidic self-assembly; movable microstructures; on-chip fabrication; photo-crosslinkable resin; poly (ethylene glycol) diacrylate; polydimethylsiloxane; yeast cells;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2012 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4673-4811-9
Type :
conf
DOI :
10.1109/MHS.2012.6492434
Filename :
6492434
Link To Document :
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