Improvement of cell capture efficiency using a reversible DEP field

Author

Chu, S.C. ; Shew, B.Y. ; Chen, C.K. ; Su, Y.C. ; Liu, S.J. ; Leng, C.H.

Author_Institution

Facility Utilization Group, Nat. Synchrotron Radiat. Res. Center, Hsinchu, Taiwan

fYear

2009

fDate

21-25 June 2009

Firstpage

1782

Lastpage

1785

Abstract

This work proposes the first time using a periodic and reversible DEP (dielectrophoresis) field to improve the capture efficiency of the rare specific cells in a mixed cell suspension. The forward DEP field will gently guide all the cells in a suspension to interact with bio-probes that are immobilized on a PVDF substrate in a reactor. The reverse DEP field will redistribute the un-bonded cells into the suspension for next reaction chance. The experimental results indicated that the forward DEP can double the cell capture efficiency compared with that without DEP enhancement, and the reversible DEP scheme can improve the efficiency about 25% further.

Keywords

biological techniques; bioreactors; cellular biophysics; electrophoresis; PVDF substrate; bioreactor; cell capture efficiency improvement; dielectrophoresis field; immobilised bioprobes; mixed cell suspension; periodic DEP field; reversible DEP field; Electrodes; Fluorescence; Immune system; Inductors; Microfluidics; Monitoring; Probes; Substrates; Synchrotron radiation; Vaccines; Specific T cell; cell counting; cell immuno-capture; fluorescence staining; reversible DEP technique;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285719

Filename

5285719