Local injection probe of functional micro-nano gel tools into Caenorhabditis elegans

Model organism is widely used in the biology and medical research fields for investigating the biological system, evaluating the drugs affectivities, and so on. The importance of model organism is 1) easy to culture, 2) well-known decoding of genes, 3) similarity with human, and so on. As model organism, we interests in Caenorhabditis elegans (C. elegans) is considered to be useful biological organism for various diseases analysis, because it is one of the smallest animal and they have many homologues genes with the human disease genes. This paper presents a novel selective nano-injection method using functional micro-nano gel tools based on nanomanipulation into the C. elegans for in-vivo local cell analysis. The functional micro-nano gel tools are fabricated to transfer the artificial material by diffusion mechanism. We assembled the gel tool at the tip of pipette as injector and evaluated the diffusion of gel capsule.