Label-free electronic detection of growth factor induced cellular chatter on chemoreceptive neuron MOS (CvMOS) transistors

Author

Jayant, Krishna ; Porri, Teresa ; Erickson, Jon W. ; Kan, Edwin C.

Author_Institution

Electr. & Comput. Eng., Cornell Univ., Ithaca, NY, USA

fYear

2009

fDate

21-25 June 2009

Firstpage

1814

Lastpage

1817

Abstract

In this paper we present a novel label-free detection scheme to sense growth factor over-expression in tumor cells at the single cell level using a chemoreceptive neuron MOSFET (CvMOS). Tumor cell lines including A431, HeLa, TSE and NIH-3T3´s were dispensed onto poly-Llysine-coated sensing gates with a limited dilution and their responses to EGF (epidermal growth factor) were recorded. A change in cell shape after stimulation is observed and is validated through SEM images on witness samples. Stimulation of downstream pathways upon EGF activation is detected through an abrupt change in threshold voltage and subthreshold slope indicating cell effects from both charge exchange and capacitance.

Keywords

MOSFET; bioelectric phenomena; biosensors; capacitance; cellular biophysics; scanning electron microscopy; tumours; A431; HeLa; MOSFET; NIH-3T3; SEM images; TSE; capacitance; charge exchange; chemoreceptive neuron MOS transistors; downstream pathways; epidermal growth factor; growth factor induced cellular chatter; label-free electronic detection; polyLlysine-coated sensing gates; single cell level; subthreshold slope; threshold voltage; tumor cells; CMOS image sensors; Cancer; Capacitance; Electrodes; FETs; MOSFETs; Neurons; Scanning electron microscopy; Testing; Tumors; CMOS; Cancer; Capacitance; Cells; EGF; Growth Factor;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285727

Filename

5285727