Title :
Possibility to use iPS-technology in age-related diseases
Author :
Zhao Cheng ; Ito, Satoshi ; Nishio, Nobuhiko ; Suganya, T. ; Isobe, Keisuke
Author_Institution :
Grad. Sch. of Med., Dept. of Immunology, Nagoya Univ., Nagoya, Japan
Abstract :
When applying the iPSCs for regenerative therapy in elderly patients, it is necessary to establish the iPSCs from elderly patients themselves then differentiate the iPSCs to specific cell types for transplantation treatments. The mouse is a perfect model to study aging in mammals. Bone marrow (BM) cells from aged C57BL/6 mice (15 months to 21 months) were cultured with granulocyte macrophage-colony stimulating factor (GM-CSF). The efficiency to produce iPSCs from aged mice BM cells was lower than that of young mice. We established several clones of iPSCs from aged mice. All the established clones have pluripotent markers. Aged-iPSCs could differentiate to three germ layers in vitro and made teratoma in vivo. We are currently making chimeric mice between Aged-iPSC-1 and ICR mice.
Keywords :
bone; cellular biophysics; diseases; gene therapy; geriatrics; molecular biophysics; tissue engineering; C57BL-6 mice; GMCSF; ____________; age 1.25 yr to 1.75 yr; age related diseases; aged ICR mice; aged iPSC-1 mice; bone marrow cells; chimeric mice; clone pluripotent markers; elderly patients; granulocyte macrophage colony stimulating factor; iPS technology; iPSC clones; iPSC differentiation; in vitro germ layers; in vivo teratoma; mammal aging; mouse model; regenerative therapy; specific cell types; transplantation treatments;
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2012 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4673-4811-9
DOI :
10.1109/MHS.2012.6492465
