Title :
Subcellular-resolution recording of electrical activity using a CMOS-microelectroode system
Author :
Frey, U. ; Egert, U. ; Heer, F. ; Hafizovic, A. ; Hierlemann, A.
Author_Institution :
Dept. of Biosystems Sci. & Eng., ETH Zurich, Basel, Switzerland
Abstract :
A high-density planar microelectrode system (HD-MEA), based on complementary metal-oxide-semi-conductor (CMOS) microelectronics technology has been used for in vitro recordings of extracellular potentials. At the same time, subcelllular lateral resolution and high temporal resolution (sampling at 20 kHz) of cellular electrical activity have been achieved at unprecedented signal quality (noise levels as low as7-9muVRMS. The system has been applied to various samples, amongst those slices, and the temporal evolution of single-cell action potential has been studied in detail. The signals of a single cell were visible on more than 50 of the tightly spaced electrodes.
Keywords :
CMOS integrated circuits; bioelectric phenomena; biomedical electrodes; cellular biophysics; CMOS-microelectroode system; HD-MEA; Purkinje cells; cellular electrical activity; complementary metal-oxide-semi-conductor; extracellular potentials; high temporal resolution; high-density planar microelectrode system; in vitro recordings; microelectrode array; microelectronics technology; subcelllular lateral resolution; subcellular-resolution recording; CMOS technology; Circuits; Electrodes; Extracellular; In vitro; Microelectrodes; Neurons; Passivation; Signal resolution; Spatial resolution; CMOS; Microelectrode array; Purkinje cells; acute slices; neurons; subcellular resolution;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285753
