Subcellular-resolution recording of electrical activity using a CMOS-microelectroode system

Author

Frey, U. ; Egert, U. ; Heer, F. ; Hafizovic, A. ; Hierlemann, A.

Author_Institution

Dept. of Biosystems Sci. & Eng., ETH Zurich, Basel, Switzerland

fYear

2009

fDate

21-25 June 2009

Firstpage

1674

Lastpage

1677

Abstract

A high-density planar microelectrode system (HD-MEA), based on complementary metal-oxide-semi-conductor (CMOS) microelectronics technology has been used for in vitro recordings of extracellular potentials. At the same time, subcelllular lateral resolution and high temporal resolution (sampling at 20 kHz) of cellular electrical activity have been achieved at unprecedented signal quality (noise levels as low as7-9muV_RMS. The system has been applied to various samples, amongst those slices, and the temporal evolution of single-cell action potential has been studied in detail. The signals of a single cell were visible on more than 50 of the tightly spaced electrodes.

Keywords

CMOS integrated circuits; bioelectric phenomena; biomedical electrodes; cellular biophysics; CMOS-microelectroode system; HD-MEA; Purkinje cells; cellular electrical activity; complementary metal-oxide-semi-conductor; extracellular potentials; high temporal resolution; high-density planar microelectrode system; in vitro recordings; microelectrode array; microelectronics technology; subcelllular lateral resolution; subcellular-resolution recording; CMOS technology; Circuits; Electrodes; Extracellular; In vitro; Microelectrodes; Neurons; Passivation; Signal resolution; Spatial resolution; CMOS; Microelectrode array; Purkinje cells; acute slices; neurons; subcellular resolution;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285753

Filename

5285753