Title :
Putting motile bacteria to work
Author_Institution :
Rowland Inst., Cambridge, MA, USA
Abstract :
Bacteria swim by exploiting the frictional drag forces encountered in their low Reynolds number environment. This is a world in which viscous forces overwhelm inertial forces. Designers of microscale hydrodynamic devices work in a similar realm. So we ask whether motile bacteria can be employed to solve some of the problems encountered in microfluidic devices. When deposited on a surface at a fluid interface, might they produce useful flows? When deposited on the surface of a polystyrene bead, might they move that bead around? We show movies of fluorescent motile bacteria and the movement of their flagella. We describe a robust and simple technique for attaching Serratia marcescens to polydimethylsiloxane (used in soft micro-fluidic devices) and to polystyrene beads (as sample objects for bacterial transport). Finally, we present evidence demonstrating enhanced flow and motion.
Keywords :
biomechanics; fluidic devices; fluorescence; friction; hydrodynamics; microfluidics; microorganisms; plastic flow; polymers; Serratia marcescens; bacterial transport; flagella movement; fluid interface; fluorescent motile bacteria; frictional drag forces; low Reynolds number environment; microscale hydrodynamic devices; polydimethylsiloxane; polystyrene bead; soft micro-fluidic devices; viscous forces; Adhesives; Biological system modeling; Biomedical signal processing; Biosensors; Circuit noise; Circuit simulation; Microorganisms; Silicon; Switches; Transducers;
Conference_Titel :
Bio-, Micro-, and Nanosystems, 2003. ASM Conferences
Print_ISBN :
1-55581-279-3
DOI :
10.1109/BMN.2003.1220614