Title :
Multiplexed analysis of inflammation biomarkers using spectrally-encoded on-chip electrophoresis
Author :
Hou, Chenlu ; Herr, Amy E.
Author_Institution :
Univ. of California, Berkeley, CA, USA
Abstract :
We report on development of spectrally multiplexed immunoassays for inflammatory biomarkers utilizing onchip electrophoresis. Direct immunoassays are developed for TNF-alpha and CRP using affinity reagents (antibodies) labeled with spectrally distinct fluorophores. Following off chip incubation, unbound affinity reagents were rapidly resolved from bound immune complex via on-chip electrophoretic separation. Bound immune complex were quantified using 2-color imaging. Dose response curves are generated for both biomarkers at clinically relevant concentrations. With spectral multiplexing, multiple biomarkers can be analyzed in a single microfluidic channel. The technique reported here shows promise as biomarker profiling tool for differential diagnosis in clinical setting.
Keywords :
biochemistry; biomedical imaging; dosimetry; electrophoresis; microfluidics; molecular biophysics; patient diagnosis; proteins; tumours; 2-color imaging; C-reactive protein; affinity reagents; antibodies; bound immune complex; clinical diagnosis; dose response; fluorophores; immunoassays; inflammation biomarkers; microfluidic channel; multiplexed analysis; off chip incubation; spectral multiplexing; spectrally-encoded on-chip electrophoresis; tumor necrosis factor; Biomarkers; Detectors; Electrokinetics; Immune system; Insulin; Microfluidics; Proteins; Routing; Throughput; Wavelength measurement; On-chip gel electrophoresis; homogeneous immunoassay; inflammatory biomarker;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285788
