Title :
Computational model of DIC microscopy for reconstructing 3-D specimens: from observations to measurements
Author :
Kagalwala, F. ; Kanade, Takeo ; Lanni, F.
Author_Institution :
Robotics Inst., Carnegie Mellon Univ., Pittsburgh, PA, USA
Abstract :
Summary form only given. Differential interference contrast (DIC) microscopy, a method pioneered by Georges Nomarski, is widely used to study live biological specimens. However, to date, biologists only qualitatively interpret DIC microscope images. In this work, we describe a method to extract quantitative information from optically-sectioned DIC microscope images. Specifically, given a set of images of a specimen, we attempt to reconstruct the three-dimension structure and refractive index distribution throughout the specimen.
Keywords :
aberrations; biological techniques; image reconstruction; light interference; optical images; optical microscopy; ray tracing; refractive index; 3D structure reconstruction; Nomarski microscopy; aberrations; computational model; differential interference contrast microscopy; generalised ray tracer; interference image; iterative nonlinear optimisation; live biological specimen; optically sectioned microscope images; prism parameters; quantitative information; refractive index distribution; Biological system modeling; Biology computing; Biomedical optical imaging; Computational modeling; Data mining; Image reconstruction; Interference; Microscopy; Optical refraction; Optical variables control;
Conference_Titel :
Lasers and Electro-Optics, 1999. CLEO '99. Summaries of Papers Presented at the Conference on
Conference_Location :
Baltimore, MD, USA
Print_ISBN :
1-55752-595-1
DOI :
10.1109/CLEO.1999.833909
